Figure 4

Overexpression of AtUCP1 increased mitochondrial biogenesis through donut formation/fragmentation. (A) Representative TEM micrograph of WT L2 leaves. (B) Representative TEM micrograph of P07 L2 leaves. (C) Quantification of the mitochondrial number, area, and total area occupied by mitochondria in TEM micrograph field images of WT and P07 plants. (D) Close-up of the donut-shaped mitochondria at different developmental stages in P07 transgenic cells. Image analysis was performed on 20 field images (n = 20) for each genotype. *p < 0.05 compared with WT. Error bars, mean ± s.e.m. Scale bars, 1 μm.