Figure 1

Transgenic tobacco overexpressing AtUCP1 exhibits a normal plant phenotype independent of the UCP1 expression level. (A) Wild-type (WT) and AtUCP1 transgenic lines P49 and P07 grown for 12 weeks in 50:50 soil/vermiculite mixtures fertilized with half-strength MS medium not supplemented with sucrose. (B) Transcript abundance of AtUCP1 in L1 and L3 leaves of WT and transgenic lines P49 and P07. (C) Immunoblot analysis of L3 leaves of WT and transgenic lines P49 and P07 using an anti-AtUCP1 antibody. The loading control was examined using an anti-β-actin antibody. Panel (A) is representative of an experiment using 12 plants for each genotype (n = 12). Panel (B) is representative of an experiment with 6 plants for each genotype (n = 6). Panel (C) is representative of an experiment conducted in triplicate. *p < 0.05 compared with the control. Error bars, mean ± s.e.m.