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Table 4 Fatty acid composition of selected soybean tissues derived from hydroponically grown 4-week old SACPD-C mutant lines and wild-type lines grown in controlled growth chambers

From: Deletions of the SACPD-C locus elevate seed stearic acid levels but also result in fatty acid and morphological alterations in nitrogen fixing nodules

 

1mean % fatty acid of total ± one standard deviation

Tissue

Genotype

Mutation

n=

C16:0

C18:0

C18:1∆9

2C18:1∆11

C18:2

C18:3

Nodules

W82

WT

5

18.8 ± 0.4

A

4.4 ± 0.7

A

3.7 ± 0.8

A

25.6 ± 2

A

31.3 ± 0.5

A

16.6 ± 0.4

A

FN8-10

ΔSACPD-C

5

13.2 ± 0.5

B

11.7 ± 0.8

B

0.9 ± 0.3

C

26.4 ± 0.5

A

28.3 ± 0.7

C

19.9 ± 1.2

B

194D

V211E

4

13.9 ± 1.3

BC

13.5 ± 1.3

B

1.3 ± 0.8

BC

20.8 ± 1.2

B

28.8 ± 0.9

C

22.1 ± 1.2

C

Dare

WT

5

19.9 ± 0.4

A

4.1 ± 0.6

A

2.1 ± 0.8

B

26.1 ± 1.1

A

33.4 ± 1.3

B

14.7 ± 0.5

D

FAM94-41

D126N

5

14.9 ± 0.2

C

12.6 ± 1.2

B

1.1 ± 0.4

BC

25.8 ± 1

A

31.4 ± 0.6

A

14.4 ± 0.6

D

Roots

W82

WT

5

26.1 ± 0.9

A

10.2 ± 2.2

A

4.9 ± 0.6

AB

1.7 ± 1.3

A

35.8 ± 1.8

A

21.6 ± 1.6

A

FN8-10

ΔSACPD-C

5

26.7 ± 0.6

A

12 ± 1.7

AB

5.5 ± 1.3

A

0.9 ± 0.2

A

34.1 ± 1.5

AB

21.1 ± 1.9

A

194D

V211E

4

29 ± 1

B

14.2 ± 1.2

B

5.3 ± 0.8

AB

1.4 ± 0.7

A

31.4 ± 0.8

B

19 ± 0.6

A

Dare

WT

5

26.8 ± 1.3

A

11.1 ± 2.3

AB

4.1 ± 0.8

AB

0.9 ± 0.1

A

35.5 ± 2

A

21.9 ± 2.6

A

FAM94-41

D126N

5

27.3 ± 1

AB

13.2 ± 2.1

AB

3.5 ± 0.8

B

1.2 ± 0.8

A

35 ± 2.4

AB

20.1 ± 1.9

A

Leaves

W82

WT

5

13.8 ± 0.5

A

5.2 ± 0.4

AB

2.1 ± 0.2

A

ND2

13.7 ± 0.7

A

65.4 ± 0.8

A

FN8-10

ΔSACPD-C

5

14.4 ± 0.8

AB

5.3 ± 0.4

AB

2.2 ± 0.6

A

ND

14.5 ± 1.7

A

63.9 ± 2.8

A

194D

V211E

4

14.3 ± 0.3

AB

6 ± 0.3

C

2.2 ± 0.3

A

ND

15.3 ± 1.3

A

62.5 ± 1.8

A

Dare

WT

5

14.4 ± 0.3

AB

5.5 ± 0.2

AC

2.3 ± 0.6

A

ND

13.7 ± 1.4

A

64.4 ± 2.1

A

 

FAM94-41

D126N

5

14.8 ± 0.3

B

4.9 ± 0.2

B

2.2 ± 0.4

A

ND

14.4 ± 1

A

64.1 ± 1.3

A

  1. Height of histogram indicates mean of 4–5 samples, and bars indicate ± one standard deviation.
  2. 1Letters adjacent to mean + standard deviation indicates result of Tukey’s HSD test (α = 0.05); common letters indicate insignificant differences.
  3. 2ND = C18:1∆11 is derived from bacteroids/bacteria and was not detected in leaf tissues.