Figure 4

Confirmed conserved and non-conserved targets of conserved miRNAs determined through degradome sequencing and RNA ligase-mediated rapid amplification of 5′ complementary DNA ends (5′ RACE). The degradome sequencing of miRNA targets is presented in the form of target plots (t-plots). The signature abundance throughout the length of the indicated transcripts is shown. The red arrows in the t-plots indicate cleavage sites (x-axis) and the detected frequencies (y-axis). The target gene (contig) names are given above the t-plots, with annotations shown in parentheses. miRNA:mRNA alignment along with the cleavage frequencies detected using 5′ RACE. The arrows indicate the 5′ ends of the miRNA-guided cleavage products, and the numbers indicate the ratios of the cleaved products of the total fragments that were sequenced.