Figure 3

Transcription patterns of maize dehydration stress responding genes under initial pre-stressed watered (W) conditions, upon the first exposure (S1), during watered recovery (R2), and upon a subsequent stress (S3) measured by real time qRT-PCR. A) Higher transcript levels in S3 than in S1 produced by [+/+] memory genes: GRMZM2G179462 encodes a putative low-temperature ABA-induced protein integral to the plasma membrane; GRMZM2G053669 encodes a putative asparagine synthase; GRMZM2G011598 encodes a NAC domain protein involved in hypersensitive response; B) lower transcripts in S3 than in S1 produced by [-/-] memory genes: GRMZM2G306104 encodes a putative plastid gene; GRMZM2G151365 encodes unknown protein; GRMZM2G303993 encodes putative RNA-helicase; C) genes induced in S1 but revising response in S3 [+/-] memory genes: GRMZM2G390804 encodes a protein with unknown function; GRMZM2G012453 encodes a putative microtubule organizing protein; GRMZM2G013170 encodes a putative disease resistance protein from the RPP family; D) genes repressed in S1 but regaining activity in R1 and maintaining it in S3 [-/+] memory genes: GRMZM2G082830 encodes a putative membrane-associated kinase; GRMZM2G068519 encodes a putative Flavin-binding oxygenase; GRMZM2G110192 is an NCED4 homolog; E) and F) genes repetitiously providing similar transcript amounts during each exposure to the dehydration stress: [+/=] GRMZM2G159700 and GRMZM2G026892 encoding a protein of the cupin-superfamily and an unknown protein, respectively. The [-/=] nontrainable genes GRMZM2G055973 and GRMZM2G467576 encode putative Zn-finger proteins from the RING family.