Skip to main content

Table 1 Levels of defense-related enzymes in tomato plants in response to six treatments

From: Secretions from the ventral eversible gland of Spodoptera exigua caterpillars activate defense-related genes and induce emission of volatile organic compounds in tomato, Solanum lycopersicum

Hours after treatment Treatment Enzymatic activity (nkat/mg protein)
POD PPO LOX
0 VEGI 55.29 ± 2.95a 52.35 ± 4.65a 13.54 ± 0.66a
  VEGA 61.17 ± 1.35a 58.23 ± 0.89a 28. 45 ± 6.54a
  MI + OSVEGI 66.46 ± 1.14a 56.42 ± 2.80a 22.71 ± 1.12a
  MI + OSVEGA 64.60 ± 4.38a 62.39 ± 11.44a 11.20 ± 0.88a
  MI 49.70 ± 0.75a 59.25 ± 5.49a 12.97 ± 3.48a
  Undamaged 56.48 ± 1.14a 60.51 ± 3.47a 34.80 ± 2.14a
24 VEGI 363.60 ± 17.66a 212.08 ± 20.20b 85.84 ± 12.59a
  VEGA 182.81 ± 3.97c 130.06 ± 8.76c 57.67 ± 3.22b
  MI + OSVEGI 239.45 ± 24.23b 236.78 ± 23.97a 127.54 ± 6.61a
  MI + OSVEGA 175.78 ± 5.79c 139.45 ± 4.65c 46.56 ± 1.71b
  MI 179.29 ± 21.89c 134.82 ± 25.09c 53.33 ± 5.24b
  Undamaged 178.70 ± 1.22c 141.43 ± 6.76c 22.05 ± 0.61b
48 VEGI 407.96 ± 17.10b 305.29 ± 17.47a 70.75 ± 2.06a
  VEGA 157.79 ± 10.50d 125.63 ± 22.31c 33.95 ± 2.89d
  MI + OSVEGI 489.27 ± 23.70a 210.60 ± 7.35b 57.06 ± 2.70b
  MI + OSVEGA 188.80 ± 11.67c 129.27 ± 6.05c 17.17 ± 2.22c
  MI 199.16 ± 10.30c 89.65 ± 68.88c 31.18 ± 5.37d
  Undamaged 195.25 ± 7.41c 89.63 ± 17.64c 27.47 ± 6.49d
72 VEGI 552.26 ± 24.75a 339.99 ± 21.92a 213.67 ± 35.32a
  VEGA 186.40 ± 7.67e 130.76 ± 24.01c 63.78 ± 8.84b
  MI + OSVEGI 492.27 ± 17.01b 246.12 ± 15.96b 219.46 ± 9.81a
  MI + OSVEGA 224.92 ± 12.97c 143.74 ± 8.34c 65.38 ± 3.07b
  MI 150.80 ± 8.22d 137.80 ± 5.03c 70.18 ± 1.85b
  Undamaged 187.66 ± 18.53e 147.32 ± 20.83c 69.45.68 ± 1.09b
  1. Table shows activity (expressed as mean ± SEM nkat/mg protein) of three defense-related enzymes, peroxidase (POD), polyphenol oxidase (PPO), and lipoxygenase (LOX), in tomato plants damaged by caterpillars with the VEG intact (VEGI), plants damaged by caterpillars with the VEG ablated (VEGA), mechanically injured (MI) plants, mechanically injured plants treated with oral secretion (OS) from VEGI caterpillars (MI + OSVEGI), mechanically injured plants treated with OS from VEGA caterpillars (MI + OSVEGA), and undamaged (control) plants, at 0, 24, 48 and 72 h after caterpillar feeding. Data were collected from three plants (i.e. 3 biological replicates) per treatment. Means (±SEM) within the same column and time period having different letters are significantly different (P < 0.05).