|
Hours after treatment
|
Treatment
|
Enzymatic activity (nkat/mg protein)
|
|---|
|
POD
|
PPO
|
LOX
|
|---|
|
0
|
VEGI
|
55.29 ± 2.95a
|
52.35 ± 4.65a
|
13.54 ± 0.66a
|
| |
VEGA
|
61.17 ± 1.35a
|
58.23 ± 0.89a
|
28. 45 ± 6.54a
|
| |
MI + OSVEGI
|
66.46 ± 1.14a
|
56.42 ± 2.80a
|
22.71 ± 1.12a
|
| |
MI + OSVEGA
|
64.60 ± 4.38a
|
62.39 ± 11.44a
|
11.20 ± 0.88a
|
| |
MI
|
49.70 ± 0.75a
|
59.25 ± 5.49a
|
12.97 ± 3.48a
|
| |
Undamaged
|
56.48 ± 1.14a
|
60.51 ± 3.47a
|
34.80 ± 2.14a
|
|
24
|
VEGI
|
363.60 ± 17.66a
|
212.08 ± 20.20b
|
85.84 ± 12.59a
|
| |
VEGA
|
182.81 ± 3.97c
|
130.06 ± 8.76c
|
57.67 ± 3.22b
|
| |
MI + OSVEGI
|
239.45 ± 24.23b
|
236.78 ± 23.97a
|
127.54 ± 6.61a
|
| |
MI + OSVEGA
|
175.78 ± 5.79c
|
139.45 ± 4.65c
|
46.56 ± 1.71b
|
| |
MI
|
179.29 ± 21.89c
|
134.82 ± 25.09c
|
53.33 ± 5.24b
|
| |
Undamaged
|
178.70 ± 1.22c
|
141.43 ± 6.76c
|
22.05 ± 0.61b
|
|
48
|
VEGI
|
407.96 ± 17.10b
|
305.29 ± 17.47a
|
70.75 ± 2.06a
|
| |
VEGA
|
157.79 ± 10.50d
|
125.63 ± 22.31c
|
33.95 ± 2.89d
|
| |
MI + OSVEGI
|
489.27 ± 23.70a
|
210.60 ± 7.35b
|
57.06 ± 2.70b
|
| |
MI + OSVEGA
|
188.80 ± 11.67c
|
129.27 ± 6.05c
|
17.17 ± 2.22c
|
| |
MI
|
199.16 ± 10.30c
|
89.65 ± 68.88c
|
31.18 ± 5.37d
|
| |
Undamaged
|
195.25 ± 7.41c
|
89.63 ± 17.64c
|
27.47 ± 6.49d
|
|
72
|
VEGI
|
552.26 ± 24.75a
|
339.99 ± 21.92a
|
213.67 ± 35.32a
|
| |
VEGA
|
186.40 ± 7.67e
|
130.76 ± 24.01c
|
63.78 ± 8.84b
|
| |
MI + OSVEGI
|
492.27 ± 17.01b
|
246.12 ± 15.96b
|
219.46 ± 9.81a
|
| |
MI + OSVEGA
|
224.92 ± 12.97c
|
143.74 ± 8.34c
|
65.38 ± 3.07b
|
| |
MI
|
150.80 ± 8.22d
|
137.80 ± 5.03c
|
70.18 ± 1.85b
|
| |
Undamaged
|
187.66 ± 18.53e
|
147.32 ± 20.83c
|
69.45.68 ± 1.09b
|
- Table shows activity (expressed as mean ± SEM nkat/mg protein) of three defense-related enzymes, peroxidase (POD), polyphenol oxidase (PPO), and lipoxygenase (LOX), in tomato plants damaged by caterpillars with the VEG intact (VEGI), plants damaged by caterpillars with the VEG ablated (VEGA), mechanically injured (MI) plants, mechanically injured plants treated with oral secretion (OS) from VEGI caterpillars (MI + OSVEGI), mechanically injured plants treated with OS from VEGA caterpillars (MI + OSVEGA), and undamaged (control) plants, at 0, 24, 48 and 72 h after caterpillar feeding. Data were collected from three plants (i.e. 3 biological replicates) per treatment. Means (±SEM) within the same column and time period having different letters are significantly different (P < 0.05).
