Figure 9

Expression of stress-responsive genes in wild-type and transgenic plants subjected to cold stress. Relative expression levels of stress-responsive genes were determined by qRT-PCR using cDNA synthesized from total RNAs isolated from the leaves of 2-week-old Arabidopsis exposed to low temperature 4°C for 48 h. A, RD29A; B, RAB18; C, RD22; D, ERD15; E, KIN1. There were four treatments: WT CK represents wild-type plants grown under non-stressed conditions; 35S::CaMBF1 CK represents transgenic plants grown under non-stressed conditions; 35S::CaMBF1 Cold represents transgenic plants subjected to cold stress; WT Cold represents wild-type plants subjected to cold stress. Arabidopsis elF4A gene (At3g13920) was used as an internal control for normalization of different cDNA samples. The expression levels of stress-responsive genes in wild-type plants at 0 h were used as control (quantities of calibrator) and were assumed as 1. Three biological triplicates were averaged and Bars indicate standard error of the mean.