Figure 3

Absolute quantification of alternatively spliced RNA variants. Ten-day-old Col-0 plants grown on MS-agar plates were used for the extraction of total RNA samples. To maximize the sensitivity of detection, plants were harvested at the phase of peak expression for each gene. A series of 10-fold dilutions of plasmid DNA containing each gene sequence was used to generate a standard curve. The regression line from the dilution curve was used to determine the concentration of each RNA splice variant. Black circles represent the absolute amounts of RNA splice variants (A). C_T, threshold cycle. The percentages of β/α + β were calculated for each clock gene (B).