Figure 2

Detection of RNA splice variants of the clock genes. A. Detection of RNA splice variants by RT-PCR. Total RNA samples were isolated from 10-day-old Col-0 plants grown on MS-agar plates under LDs at peak ZT point for each clock gene and subject to RT-PCR. Gene-specific F and R primer sets, as indicated in Figure 1, were used to detect the transcript isoforms of each clock gene. PCR reactions were also performed without reverse transcription (-RT) to verify the lack of genomic DNA contamination. The sizes of the PCR products are provided at the bottom of the figure. SM, DNA size marker. bp, base pair. B. Modes of splicing events. Detection methods for the alternative splicing events are listed in the 3^rd column with the references indicated in the 4^th column. The nucleotide sequences of the RNA splice variants were determined (This work) or verified by direct DNA sequencing in this work. RNA-seq, RNA sequencing. Sanger, DNA sequencing by Sanger method.