Figure 3

WLR, RWC, soluble sugars, proline, and stomatal status of OsCPK9 -OX and OsCPK9 -RNAi transgenic lines. (A) WLR, RWC, soluble sugars, and proline contents of OsCPK9-OX and OsCPK9-RNAi transgenic lines. (B) Scanning electron microscope images of stomatal status; open, closed, partially open. (C) Proportions of open, closed, and partially open stomata. Leaves of 3-week-old rice seedlings were collected to determine the WLR and RWC of control plants and transgenic lines. Three-week-old rice seedlings were deprived of water for 15 days and then soluble sugars, proline and stomatal status were examined with leaf samples. Data are means ± SE of four independent experiments for (A) and three independent experiments for (C). Asterisks indicate significant difference between the WT and transgenic lines (*p <0.05; **p <0.01).