Figure 2

Changes in sulfate uptake capacity of Brassica juncea roots. Plants were exposed to different Cd concentrations for 48 h (A, C) or grown under different sulfate concentrations for 10 days (B, D). (A, B) Sulfate influxes were evaluated by measuring the rate of ³⁵SO₄ ^2- absorption into roots of intact plants over a 15 min pulse. The incubation solutions contained 200 μM SO₄ ^2-. Bars and error bars are means and SE of three experiments run in triplicate (n = 9). Different letters indicate significant differences (P < 0.05). (C, D) Semi-quantitative RT-PCR analysis of BjSultr1;1 and BjSultr1;2 gene expression. PCRs were carried out for 24 cycles where cDNAs were exponentially amplified. For BjSultr1;2 pool, primers were designed on conserved sequences of the three BjSultr1;2 variants, and gave overlapping amplification products of 1046 bp. PCR products were separated in agarose gel and stained with SYBR Green I. Signals were detected using a laser scanner with 532 nm laser and 526 nm filter. BjTub, tubulin. A representative set of data from three independent experiments is given.