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Figure 4 | BMC Plant Biology

Figure 4

From: Growth attenuation under saline stress is mediated by the heterotrimeric G protein complex

Figure 4

Promoting the active state of G signaling by disfavoring heterotrimer formation confers salt tolerance. Col-0, agb1-2, and Arabidopsis seedlings overexpressing (35S promoter) mutated versions of the AGB1 gene in the agb1-2 background were germinated and grown in ¼ MS plates supplement with 100 mM NaCl. A) Green seedlings were scored 10 d after germination. Mutants are indicated with the wild-type residue position and substitution. Mutations W109A and S129A both lie in the Gα:Gβγ protein interface and thus shift the equilibrium away from heterotrimer formation to active Gα subunit and Gβγ dimer. Data is expressed as the mean of 3 replicates with 36 seedlings per genotype per plate; error bars = SD. Pair wise comparisons between the means of Col 0 plants complemented with mutant variants of the AGB1 genes were performed with a single-tailed T-test. *, means comparison show up as statistically significant. The agb1-2 null mutation conferred reduced number of green seedling (CL = 99%, p value 0.0009). Plants complemented with the W109A or S129A variants of the AGB1 gene produced higher number of green seedlings, (CL = 99%, p value 0.0042 and CL = 90%, p value 0.07), respectively). B) Images of seedling were taken at the end of the treatment. Two lines for agb1-2 seedling complemented with mutated AGB1 are shown.

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