Figure 3

Genetic characterization of G-protein signaling under hypersaline/hyperosmotic stress. Arabidopsis seeds from each genotype were germinated and seedlings grown on ¼ MS agar media supplemented with 100 mM NaCl. Green seedlings were counted 10 d after germination. Error bars represent the standard deviation calculated from triplicates. Pair wise comparisons between the means were done with t-tests at a confidence level (CL) of 90% and 95%. “a” indicates that the mean for rgs1-2 differs from Col-0 or rgs1-2/agb1-2 double mutant (CL 95%, p-values = 0.002 and 0.005 respectively). “b” indicates gpa1-4 and Col 0 have different means (CL 95%, p-value = 0.02). Results are representative of three different experiments. “c” indicates that the means of these genotypes were compared with all the other genotypes and always resulted in statistical significant differences between them and all other genotypes not denoted with “c” at a CL of 95% (p value < 0.05). “d” indicates that agg1-1 and agg2-1 have different means (CL 90%, p-value = 0.051).