Figure 4

Response of pepper leaves (A) and tobacco leaves (B) individually inoculated with A. tumefaciens harboring PVX vector containing various site-directed mutations of PcNLP 1. Accession numbers are PcNLP1, PcNLP1D112A, PcNLP1H120A, PcNLP1D123A, PcNLP1E125A, and PcNLP1D112A/H120A/D123A/E125A. Each leaf was co-inoculated with PcINF1 at symmetric sites as positive control. The empty PVX vector and distilled water were used as negative controls. Circles indicate the disservice regions around the point of inoculation. Photographs show individual, representative leaves taken at 10 dpi. The experiments were repeated at least three times.