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Figure 3 | BMC Plant Biology

Figure 3

From: Characterization of necrosis-inducing NLP proteins in Phytophthora capsici

Figure 3

Comparative analysis of chlorosis-inducing or necrosis response observed for various PcNLP genes by PVX agroinfection in tobacco leaves. A: Leaves were inoculated with A. tumefaciens harboring the PVX vector containing various PcNLP genes. Accession numbers are PcNLP1, PcNLP2, PcNLP3, PcNLP6, PcNLP7, PcNLP8, PcNLP9, PcNLP10, PcNLP13, PcNLP14, and PcNLP15. Each leaf was co-inoculated with PcINF1 at symmetric sites as positive control. The empty PVX vector and distilled water were used as negative controls on the same leaf. Circles indicate the disservice regions around the point of inoculation. Photographs show individual, representative leaves taken at 10 dpi. The experiments were repeated at least three times. B: Western blot analysis of each PcNLP gene secreted following PVX vector agroinfection in tobacco leaves. The proteins of agroinfiltrated leaves expressing PcINF1 with HA-tag and each PcNLP gene secreted from strain SD33 were used as positive controls. The total proteins of wild type leaves were used as negative control. a: The protein extracted from lesions spots; b: The protein extracted from strain SD33.

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