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Table 7 Glucosinolate profiles in leaves of Arabidopsis mutants Atcyp81f4 transformed with the respective expression vector constructs

From: Functional identification of genes responsible for the biosynthesis of 1-methoxy-indol-3-ylmethyl-glucosinolate in Brassica rapa ssp. chinensis

Mutant lines

Expression construct

3MSOP

4MSOB

4MTB

5MSOP

8MSOO

4OHI3M

I3M

4MOI3M

1MOI3M

M3-1

Control

0.92 ± 0.08

6.26 ± 0.96

0.93 ± 0.17

0.16 ± 0.02

0.44 ± 0.08

0.05 ± 0.01

1.40 ± 0.19

0.37 ± 0.04

0.00 ± 0.00

M3-6

Control

0.90 ± 0.22

6.22 ± 1.54

0.89 ± 0.37

0.18 ± 0.05

0.50 ± 0.14

0.04 ± 0.01

1.25 ± 0.23

0.34 ± 0.04

0.00 ± 0.00

M3-1

35S::BrCYP81F4-1

0.90 ± 0.18

6.16 ± 1.54

0.80 ± 0.11

0.17 ± 0.04

0.42 ± 0.12

0.04 ± 0.01

1.21 ± 0.16

0.32 ± 0.04

0.08 ± 0.01*

M3-6

35S::BrCYP81F4-1

0.99 ± 0.03

7.67 ± 0.62

0.64 ± 0.09

0.21 ± 0.02

0.42 ± 0.07

0.02 ± 0.01

1.59 ± 0.22

0.34 ± 0.06

0.09 ± 0.03*

M3-1

35S::BrCYP81F4-2

0.86 ± 0.07

6.47 ± 1.07

1.01 ± 0.61

0.19 ± 0.04

0.66 ± 0.41

0.00 ± 0.00*

0.64 ± 0.35*

0.27 ± 0.01*

0.97 ± 0.64*

M3-6

35S::BrCYP81F4-2

0.67 ± 0.13

4.71 ± 0.90

0.57 ± 0.05

0.13 ± 0.03

0.28 ± 0.06*

0.01 ± 0.01*

0.43 ± 0.09*

0.20 ± 0.04*

0.86 ± 0.40*

  1. 3MSOP, 3-methylsulfinyl-propyl GS; 4MSOB, 4-methylsulfinyl-butyl GS; 4MTB, 4-methylthio-butyl GS; 5MSOP, 5-methylsulfinyl-pentyl GS; 8MSOO, 8-methylsulfinyl-octyl GS; 4OHI3M, 4-hydroxy-indol-3-ylmethyl GS; I3M, indol-3-ylmethyl GS; 4MOI3M, 4-methoxy-indol-3-ylmethyl GS; 1MOI3M, 1-methoxy-indol-3-ylmethyl GS. Values represent the mean ± standard deviation of three to six individual plants homozygous for the respective T-DNA insertion and transformed with the respective expression constructs. Significant differences to respective control tissue (P < 0.05) as determined using unpaired two-tailed t-test, are marked with an asterisk. Values are given in μmol * g-1 dry weight. Control, vector control pK7WG2; 35S::BrCYP81F4-1, expression construct using destination vector pK7WG2 recombined with BrCYP81F4-1; 35S::BrCYP81F4-2, expression construct using destination vector pK7WG2 recombined with BrCYP81F4-2.