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Table 5 Glucosinolate content in Arabidopsis mutants 48 hours after application of methyl jasmonate

From: Functional identification of genes responsible for the biosynthesis of 1-methoxy-indol-3-ylmethyl-glucosinolate in Brassica rapa ssp. chinensis

Mutant

Treatment

Total aliphatic GS

I3M

4MOI3M

1MOI3M

Total indol GS

Total GS

Atcyp81f4

500 μM MeJA

71 ± 21

86 ± 30

162 ± 12*

-*

87 ± 23

75 ± 21

Atcyp81f1

500 μM MeJA

83 ± 10

52 ± 5*

257 ± 17*

110 ± 10

81 ± 5

83 ± 7

Atcyp81d1

200 μM MeJA

86 ± 30

98 ± 6

72 ± 6

98 ± 50

95 ± 5

86 ± 26

  1. Total aliphatic GS, 3-methylsulfinyl-propyl GS; 4-methylsulfinyl-butyl GS; 4-methylthio-butyl GS; 5-methylsulfinyl-pentyl GS; 8-methylsulfinyl-octyl GS. Total indol GS, I3M, indol-3-ylmethyl GS; 4MOI3M, 4-methoxy-indol-3-ylmethyl GS; 1MOI3M, 1-methoxy-indol-3-ylmethyl GS. Values represent the mean ± standard deviation of three individual plants homozygous for the respective T-DNA insertion. Significant differences to the respective control treatment (P < 0.05) as determined using unpaired two-tailed t-test, are marked with an asterisk. Values are given in% on dry matter basis of the respective treatment of control plants. -, below detection limit in mutant.