Functional identification of genes responsible for the biosynthesis of 1-methoxy-indol-3-ylmethyl-glucosinolate in Brassica rapa ssp. chinensis

Wiesner, Melanie; Schreiner, Monika; Zrenner, Rita

doi:10.1186/1471-2229-14-124

Table 3 Evaluation of expression differences upon methyl jasmonate application of Arabidopsis thaliana genes involved in glucosinolate metabolism and respective homologs of candidate genes

From: Functional identification of genes responsible for the biosynthesis of 1-methoxy-indol-3-ylmethyl-glucosinolate in Brassica rapa ssp. chinensis

*Arabidopsis*gene, encoded protein	Induction	Related pathway	*Brassica*identifier
At1g54040, ESP, epithiospecifier protein	++	GS degradation	JCVI_38382
At4g31500, SUR2, CYP83B1, chytochrom P450 monooxygenase 83B1	++	GS biosynthesis	EX039068
	++	GS biosynthesis	JCVI_109
At2g22330, CYP79B3, cytochrome P450 monooxygenase 79B3	++	GS biosynthesis	JCVI_24334
At4g39940, AKN2, APS-kinase 2	++	Sulfur assimilation	JCVI_41905
At4g39940, AKN2, APS-kinase 2	++	Sulfur assimilation	JCVI_10648
At5g14200, 3-isopropylmalate dehydrogenase	++	GS biosynthesis	JCVI_10889
At1g54020, myrosinase-associated protein	+++	GS degradation	JCVI_1353
At4g39950, CYP79B2, cytochrome P450 monooxygenase 79B2	++	GS biosynthesis	JCVI_16379
At4g39950, CYP79B2, cytochrome P450 monooxygenase 79B2	++	GS biosynthesis	JCVI_33391
At1g52040, MBP1, myrosinase-binding protein 1	+++	GS degradation	EV159250
At1g52030, MBP2, myrosinase-binding protein 2	+++	GS degradation	JCVI_2556
At1g24100, UGT74B1 UDP-glucosyl transferase 74B1	++	GS biosynthesis	JCVI_31290
At1g62540, flavin-containing monooxygenase family protein	++	GS biosynthesis	JCVI_15640
At5g25980, TGG2, glucoside glucohydrolase 2	++	GS degradation	JCVI_3890
Homologs of candidate genes, encoded proteins
At4g37410, CYP81F4, cytochrome P450 monooxygenase 81F4	+	Putative GS metabolism	JCVI_7218
At4g37410, CYP81F4, cytochrome P450 monooxygenase 81F4	+	Putative GS metabolism	EV125432
At4g35160, O-methyltransferase family 2 protein	0	Unknown	EV124048
At4g37430, CYP81F1, CYP91A2, cytochrome P450 monooxygenase 81F1	+	Putative GS metabolism	JCVI_40877
At1g13080, CYP71B2, cytochrome P450 monooxygenase 71B2	0	Putative amino acids and derivatives	JCVI_39399
At5g42590, CYP71A16, cytochrome P450 monooxygenase 71A16	+	Put. triterpene, sterole, brassinosteroide	JCVI_12863
At3g28740, CYP81D11, cytochrome P450 monooxygenase 81D11	+++	Putative phenylpro-panoid metabolism	EV170929
At5g36220, CYP91A1, CYP81D1, cytochrome P450 monooxygenase	+++	Unknown	JCVI_8990

The Genevestigator database [37] was used to evaluate expression differences of the selected genes. Arabidopsis genes homologous to the identified MeJA responsive Brassica genes are in the same order as in Table 2. Grading of changes of the log2-ratios is as follows: 0, unchanged with log2-ratio smaller 0.5; +, log2-ratio between 0.5 and 1; ++, log2-ratio between 1 and 2.5; +++, log2-ratio larger than 2.5.

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ISSN: 1471-2229

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