Metabolomic profiling of VMP1-deficient cells versus empty-vector control. (A) List of metabolites analyzed by GC-MS. The numbers represent log2 fold-change in the mutant compared to empty-vector control; positive numbers (red) denote accumulation in the mutant, negative numbers (blue): accumulation in empty-vector control. A single star (*) indicates p < 0.05; double stars (**) indicate p < 0.01. (B) Heat map of the lipids measured, sorted according to lipid class, carbon-chain length and number of double bonds. The schematic, produced by MapMan , shows the lipids' log2 fold-change in the mutant compared to empty-vector control: red circles denote lipid accumulation in the mutant, blue circles denote accumulation in empty-vector control, white circles denote nearly no change. For a comprehensive list with numeric values and statistical significance, see Additional file 4: Table S2.