Figure 1

Reannotation of two erroneously fused/split NB-LRR genes. (A) Mapping of RenSeq reads identified two distinct patterns within Solyc01g102880, suggesting a fusion of two genes (blue box); (B) In contrast, Solyc07g055380 and Solyc07g055390 are predicted individual genes (red box), however a gap-free RenSeq read coverage pattern suggested that both are part of one longer sequence. The corrected annotation was confirmed in a MAST analysis using NB-LRR specific MEME motifs (TIR, NB and LRR motifs are shown in green, red and blue boxes, respectively [10]) and are depicted as boxed arrows (green) for the novel full-length TIR-NB-LRR genes RDC0002NLR0005, RDC0002NLR0006 and RDC0002NLR0052.