RNA in situ hybridization and real-time RT PCR. (A) RNA in situ hybridization on cross sections of anthers from various anther development stages shows the locations of RNA transcripts. Signals ranged from very strong (Dmc1 premeiotic) to non-existent (GRMZM2G013331 premeiotic). Smaller inserts in the panel show controls (sense probes). (B) Real-time RT-PCR analysis of RNA from whole anthers at the premeiotic, zygotene and pollen stages. Expression level normalized with a reference gene, HMG (GRMZM5G834758), depicted as 2ΔCt. Dmc1 = coding for meiotic recombinase DMC1; Nad9 = coding for subunit of NADH-dehydrogenase, mitochondrial-encoded; RibIn = coding for putative ribosome-inactivating protein; DiDH = coding for putative dihydrolipoyl-dehydrogenase; CcmFN = coding for component of cytochrome C biogenesis, mitochondrial-encoded; RNApol = coding for putative RNA polymerase, mitochondrial-encoded.