Figure 2

Wound-induced production of ¹O ₂ in sweet potato leaves. Confocal laser-scanning microscopic (CLSM) images of sweet potato leaves. False color image of sweet potato leaf infiltrate with SOSG and exposed to light (150 μmol m^-2 s^-1 PPFD) for 15 min. (A, E) Control leaf (cK) without wounding and SOSG treatment that was kept in the dark for 12 h. (B, F) Control leaf infiltrated with SOSG and kept in dark for 15 min. (C, G) Wounded leaf (MW, HA) infiltrated with SOSG and exposed to dark for 15 min. (D, H) Wounded leaf (MW, HA) infiltrated with SOSG and exposed to light for 15mins. (I, J) Relative quantification of image inflorescence by Image J. The circle denotes the wounded area. The black arrow indicates the production of ¹O₂. The SOSG fluorescence was measured using an excitation wavelength of 525 nm. SOSG: singlet oxygen sensor green; W: wounded; cK: non-wounded.