Figure 10

Effect of E8 on ACO activity and ethylene production. (A) Ectopically overexpressed in vitro ACO activity was not altered by the addition of ectopically overexpressed E8. (B) The effect of a possible ACO activity inhibition was also evaluated in pericarp enzyme extracts, by mixing a sample with no E8 abundance and high ACO activity (left) with a sample containing a high E8 abundance and low ACO activity (middle), resulting in no inhibition of ACO activity (right). Error bars represent the standard deviation of 3 replicates. Statistical significant differences (P < 0.05) between treatments are indicated with A and B.