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Table 1 Changes in gene expression levels in response to TcLEC2 ectopic expression in leaf tissues of genes involved in various dimensions of seed development calculated from qRT-PCR measurements

From: The Theobroma cacao B3 domain transcription factor TcLEC2plays a duel role in control of embryo development and maturation

Gene Gene_ID Vector control E12Ω::TcLEC2 Fold change Gene function
TcAGL15 Tc01g040120 0.0002 ± 0.0001 0.3821 ± 0.0422 129.2 MADS box transcription factor, regulate GA biosynthesis
TcWRI1 Tc10g012790 0.0001 ± 0.0001 0.0013 ± 0.002 10.53 AP2/ERWEBP transcription factor, regulate fatty acid biosynthesis and embryo development
TcABI3 Tc01g024700 0.001 ± 0.0008 0.1355 ± 0.0103 9.57 B2, B3 domain transcription factor, regulation ABA induced gene expression
TcOLE2 Tc09g004410 0.0027 ± 0.0006 0.011 ± 0.0061 5.49 Oleosin, oily body structure protein
TcLEC1 Tc07g001180 0.0041 ± 0.003 0.433 ± 0.0133 4.14 HAP3 subunit CCAAT-binding transcription factor
TcOLE1 Tc04g001560 0.001 ± 0.0008 0.0033 ± 0.0006 3.27 Oleosin, oily body structure protein
TcKASII Tc09g006480 0.0004 ± 0.0003 0.002 ± 0.0002 -2.77 3-ketoacyl-ACP-synthase II
TcFUS3 Tc04g004970 nd nd Not Induced B3 domain transcription factor, direct bind to RY motif
TcLEC1_Like Tc06g020950 0.0011 ± 0.0006 0.0016 ± 0.0003 Not Induced HAP3 subunit CCAAT-binding transcription factor
TcVicilin Tc04g024090 0.0003 ± 0.0002 0.0002 ± 0.0001 Not Induced most abundant seed storage protein in cacao
TcBBM Tc05g019690 nd nd Not Induced AP2 transcription factor in developing embryos and seeds
TcPKL Tc09g001610 0.3973 ± 0.0578 0.3928 ± 0.0733 Not Induced CHD chromatin remodeling factor
TcWUS Tc01g001780 nd nd Not Induced Master regulator of stem cell fate determination in shoot apical meristem
TcYUC2 Tc09g009820 0.0016 ± 0.0002 0.001 ± 0.0001 Not Induced flavin monooxygenase in auxin biosynthesis
TcYUC4 Tc09g013260 0.0093 ± 0.0069 0.0085 ± 0.0021 Not Induced flavin monooxygenase in auxin biosynthesis
  1. Expression levels were normalized to TcACP1 (column 3 and 4). Fold changes were calculated as a ratio of expression levels induced by TcLEC2 relative to expression levels in tissues transformed with the vector control lacking TcLEC2 and are the mean of three biological replicates (n = 3, mean ± SE). Significance was established by t-test with p-value cut-off of 0.05.
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