Figure 7
Transient overexpression of E12Ω::TcLEC2 in IZE altered fatty acid compositions and gene expression. A. Changes of the expression levels of responsive enzymes on the fatty acid biosynthesis pathway; expression levels of genes were normalized relative to that of TcTUB1; (n = 3, mean ± SE) *represents for p-value < 0.05 by t-test. B. Molar percentages of fatty acid compositions in cacao immature zygotic embryos transiently overexpressing vector control and E12Ω::TcLEC2, respectively; (n = 3, mean ± SE). **represents for p-value < 0.001 by t-test. C. Diagram of proposed model to explain the relationship between gene expression levels and altered fatty acid compositions. Enzymes are marked in circle. Enzymes that were regulated by the activity of TcLEC2 are in black, otherwise, in grey. Abbreviation: ER, endoplasmic reticulum; ACP, acyl carrier protein; CoA, Coenzyme A; FAB2, fatty acid desaturase; Fat, fatty acyl-ACP thioesterase; KAS, 3-ketoacyl-ACP synthase; FAD2, oleoyl desaturase; FAD3, linoleoyl desaturase; FAD6, oleoyl desaturase on membrane glycerolipid; FAD7/8, linoleoyl desaturase on membrane glycerolipids; PC, phosphatidylcholine; G3P, glycerol-3-phosphate; LPA, lysophosphatidate; PA, phosphatidate; DAG, diacylglycerol; TAG, triacylglycerol; PDAT, phospholipid:diacylglycerol acyltransferase; DGAT, 1,2-sn-diacylglcyerol transferase.