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Figure 6 | BMC Plant Biology

Figure 6

From: Histone acetylation associated up-regulation of the cell wall related genes is involved in salt stress induced maize root swelling

Figure 6

The elevated H3K9 acetylation levels on ZmEXPB2 and ZmXET1 gene regions. (I) Schematic diagram of ZmEXPB2 and ZmXET1 gene regions and their amplification positions for ChIP analysis. Exons are marked by black boxes. In this study, we regarded the 5’ end sequence of the first exon as the translation start site (+1). The following cis-acting element sequences in the PLACE 26.0 database (http://www.dna.affrc.go.jp/PLACE/) are indicated as: green triangle, MYC-consensusat: (CANNTG); yellow triangle, MYB-core (CNGTTR); black triangle, ABRE-related (ACGTG);purple red triangle, CAAT-box1 (CAAT); blue triangle, TATA-box: (TATA or TATACA). N represents A, T, C or G. Bars underneath the sequence marked as A, B, C, D respectively represent the positions of the PCR-amplified regions for ChIP assay. The following regions were PCR amplified using the region-specific primer sets (Table 2). (II, III) Alterations of H3K9Ac enrichment on ZmEXPB2 (II) and ZmXET1 (III) genes after treatment of six-day-old seedlings with 200 mM NaCl for 48 h. The relative H3K9Ac levels were determined by ChIP assays using an antibody to H3K9Ac, and normalized to an internal control gene beta actin. Letters on the x-axis on each graph represent the positions of the PCR-amplified fragments (shown in I). The y-axis values represent the relative enrichment of histone modification. The relative enrichment value for each pair of primers in the control group was assigned as 1. Each experiment was repeated three times and relative acetylation levels are shown as the average values ± SD (the standard deviation). White bars, no treated seedlings; Black bars, seedlings treated with 200 mM NaCl.

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