Figure 1

Generation of transgenic rice plants producing cecropin A. A. Schematic representation of the constructs. Expression of the synthetic CecA genes was controlled by the 2.3 kb GluB1 or the 1.4 kb GluB4 promoters and the nos terminator. Relevant restriction enzyme sites for cloning purposes are indicated. B. Cecropin A accumulation in transgenic rice plants. Immunoblot analysis using anti-cecropin A antibodies of protein extracts from wild-type (WT) or transgenic seeds carrying the empty vector (EV) or the transgenes indicated. For comparative purposes, 50 ng of synthetic cecropin A was run simultaneously in the tricine-SDS gels. Lower panels show Ponceau staining of protein samples.