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Figure 7 | BMC Plant Biology

Figure 7

From: Progressive 35S promoter methylation increases rapidly during vegetative development in transgenic Nicotiana attenuata plants

Figure 7

Progression of the 35S promoter methylation over two generations. A, Mean cytosine methylation values from Figure 6 shown as trend lines over time. The methylation increase across plant development follows a similar trend in the three independent lines (ICE 4.4, PNA 1.2 and PNA 10.1). The control lines (ICE 1.1 and PNA 8.6) showed no increase in cytosine methylation over time. The generational change is denoted by the dashed line (dpg = days post germination). B, Methylation frequencies of individual positions across the generational change. Sequence wide comparison of the T2 stage (60 dpg) with the T3 stage (15 dpg) in the independent lines (ICE 4.4, PNA 1.2 and PNA 10.1). Methylation rates at individual positions were sustained across the generational transition. Positions with particular low CHH methylation frequency were indicated.

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