Figure 5

Different post-flowering photoperiod responses among Harosoy and its NILs for a determinate growth habit allele ( dt1 ) and the maturity alleles e3 and e4 in two independent experiments using different light sources, R-light–enriched [blue] and FR-light–enriched [red] conditions. In the R-light–enriched condition, a combination of fluorescent and incandescent lamps with an R:FR ratio of 1.2 was used as the light source for 16 hours after dawn followed by lighting with the same light source for an additional 4 hours. The FR-light–enriched condition involved the use of both fluorescent and incandescent lamps with an R:FR ratio of 1.2 for 16 hours after dawn followed by lighting with incandescent lamps only for an additional 4 hours. A) Number of nodes (average ± 1 standard deviation) on the main stem at 30 days after flowering. B) Number of pods (average ± 1 standard deviation) more than 3.0 cm long at 30 days after flowering. Transcripts levels of Dt1 (GmTFL1b) at stem tips in different growth stages under (C) R-light–enriched and (D) FR-light–enriched conditions. Relative transcript levels (mean and standard error; n = 3) were analyzed by quantitative RT-PCR and normalized to β-tubulin (TUB). FL indicates time of flowering.