Figure 1

Representative chiral gas chromatographic separation of enzymatic products from select recombinant lodgepole pine and jack pine monoterpene synthases that produce non-oxygenated monoterpenes as their main products. Products representing greater than 5% of the total amount are labeled. 1, (−)-α-pinene; 2, (+)-α-pinene; 3, tricyclene; 4, (−)-camphene; 5, myrcene; 6, (−)-β-pinene; 7, (+)-3-carene; 8, (−)-α-phellandrene; 9, (−)-β-phellandrene; 10, terpinolene. *GC/MS traces for PcTPS-(+)3car1, PbTPS-(+)3car1 and PbTPS-(+)3car2 also showed 5%, 12% and 7% α-terpineol respectively, which elutes later than the scale shown, but are detailed in Additional file 5: Table S5.