Figure 7
Responses in leaves of wild type (upper row) or of P25 expressing transgenic tobacco plants (lower row) caused by the infiltration of Pseudomonas syringae pv. tomato DC 3000 cell suspension (A) and detection of reactive oxygen species in untreated leaves (B). In wild type leaves (upper row) the infiltrations with P. syringae cells caused no visible reaction, or occasionally, a mechanical damaged ring at the site of the syringe contact, while in P25-epressing leaves (lower row) they caused clear HR reaction at the infiltration site (A). Detached leaves of the wt (upper row) and P25-expressing leaves (lower row) show very similar DAB and NTB-staining pattern, indicating that the transgenic leaves do not contain any additional oxidative compounds.