Physical map of AN knockout gene and phenotypic characterization of an-t1 mutant. (A) The AN gene with the positions of exons (numbered black rectangles) and introns (thick lines) are represented. The 5’ and 3’ untranslated regions are depicted in white rectangles. The location of the an-t1 T-DNA insertion is shown using an inverted black triangle. The names and locations of primers used for RT-PCR experiments are also indicated. Bar = 0. 5 kb. (B) The T-DNA insertion causes a knockout expression of the gene. The quality of the RNA and the loading control was assayed by monitoring ACTIN gene expression. (C-D) SEM images of upper developing leaves, showing a mature trichome with three branches in wild type (C) and two branches in an-t1 (D) plants. (E) Wild type and an-t1 plants at seeding stage. an-t1 is dwarf compared to WT col-0 plants. (F-G) leaf shape structures of wild type (F) and an-t1 mutant (G) are represented. Bars = 50 μm (C, D).