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Table 2 Discrimination between hemizygous and homozygous plants

From: Fast-tracking development of homozygous transgenic cereal lines using a simple and highly flexible real-time PCR assay

Sample Rint4-9/EC A NOS/EC A Rint4-9/EC ABD NOS/EC ABD EC A/EC ABD
HEM1 0.8 0.8 0.8 0.7 1.9
HEM2 0.9 0.9 1.0 0.9 2.1
HEM3 0.9 1.0 1.0 0.9 2.1
HEM4 1.1 1.0 1.2 0.9 2.0
HOM5 2.3 2.1 2.6 2.1 2.0
HOM6 1.9 2.0 2.0 1.9 2.0
HOM7 1.6 2.2 1.8 2.0 2.0
HOM8 1.8 1.7 2.2 1.8 2.3
  1. Headings indicate target regions used in ΔCT analyses, numbers refer to # copies. All comparisons gave similar results (assuming 2 copies for EC A and 6 copies for EC ABD), showing that samples 1–4 were hemizygous and 5–8 were homozygous. The last column shows the number of copies estimated for EC A using EC ABD as the reference.