Skip to main content

Table 1 PCR and real-time PCR primers

From: Fast-tracking development of homozygous transgenic cereal lines using a simple and highly flexible real-time PCR assay

Primer name

Primer sequence (5′ – 3′)

Product size (bp)

GWDcontrol_for (F)

CGCCTTCTGGCTCAACAGTTC

Endogenous gene; ca. 980

GWDcontrol_rev (R)

TATCACCTTCACCTCCACGAC

Construct: 568

JP_bx17pro5' (F)

AACCATGTCCTGAACCTTCA

606

IB_GWD3rev (R)

ATCTGTAAACCTGTCTTGTG

 

qPCR Rint4-9 F

ACATTAGCGAATAGCTGGATGAC

94

qPCR Rint4-9 R

ACATTGATATACTTAGGCACAACCT

 

qPCR NOS F

TTGAATCCTGTTGCCGGTCT

127

qPCR NOS R

GCGGGACTCTAATCATAAAAACCC

 

qPCR EC ABD F

GGGGAACACTATGGCCTCAA

79

qPCR EC ABD R

TCCAGTTGAATTATCAAGGCCA

 

qPCR EC A F

ACCTGACCTTGTAAAACCATTCAT

104

qPCR EC A R

TGACATCCTCCAACATCTCTAAC

 

qPCR Rice SPS F

AGAGATCGACGAAAAGCGGA

105

qPCR Rice SPS R

TTTTCGGGATGATCCGAGCC

 
  1. F = forward primer, R = reverse primer.