Figure 3From: Fast-tracking development of homozygous transgenic cereal lines using a simple and highly flexible real-time PCR assaySample dilution series on the four primer pairs selected for the copy number assay. R2 > 0.98, efficiency = 100 ± 5% for each dilution series. The highly similar slopes of the fitted lines (produced by regression analyses) indicate that the reaction efficiencies are comparable within the dynamic range.Back to article page