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Figure 1 | BMC Plant Biology

Figure 1

From: Fast-tracking development of homozygous transgenic cereal lines using a simple and highly flexible real-time PCR assay

Figure 1

The wheat RNAi construct showing the two targeted regions for the real-time PCR assay. To ensure that the real-time PCR primers were specific for the construct but independent of the gene of interest (GOI), the junction between the rice introns (Rint4 and 9) and the NOS terminator were targeted. The shown primer pairs were selected from 3 primer pairs tested for each location, based on specificity and efficiency.

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BMC Plant Biology

ISSN: 1471-2229

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