Expression-based and co-localization detection of arabinogalactan protein 6 and arabinogalactan protein 11 interactors in Arabidopsis pollen and pollen tubes

Table 1 Summary of confirmatory PCR assays

Gene	AGI ID	LBFC^a	FC - qPCR^b	RT-PCR^c
Ubiquitin-conjugating enzyme 20	At1g50490	+2.44	+7.0
Proline transporter 1	At2g39890	+2.02	+2.0
Zinc finger (C3HC4-type RING finger) family protein	At5g60250	+1.96	+4.0
Sucrose phosphate synthase 2 F	At5g11110	+1.70	+1.5
Expansin B5	At3g60570	+1.50	+2.0
CML42	At4g20780	+1.48	+4.5
Plant cadmium resistance 11	At1g68610	-1.99	-2.0
Cellulase 3	At1g71380	-1.98	-1.4
Phosphoinositide 4-kinase gamma 4	At2g46500	-1.75	-2.2
Heat shock protein 17.6A	At1g53540	-1.34	-2.2
CAP (Pathogenesis-related protein, putative)	At2g19970	+23.34		+
C2 domain-containing protein	At3g57880	+16.93		+
F-box family protein	At1g65760	+11.8		+
Beta glucosidase 36	At1g51490	+4.95		+
CAP (allergen V5)	At2g19980	+4.79		+
Hypothetical protein	At2g22340	-141.79		-
Pentatricopeptide (PPR)	At5g28380	-44.55		-
Avirulence-responsive family protein	At4g09950	-25.77		-
DET3	At1g12840	-12.71		-

^a Lower bound of fold change (microarray experiment).
^bagp6 agp11 to wild-type fold change for qPCR experiments.
^c Genes up- or down-regulated in agp6 agp11, as assessed by semi-quantitative RT-PCR are indicated with a (+) or a (−) sign, respectively.

ISSN: 1471-2229