Table 1 Forward and reverse primers for peach genes used in qPCR analysis of biosynthetic enzymes and candidate transcription factors
Gene | Accession No./Prunus gene model | Real time PCR primers (5’ to 3’) | |
|---|---|---|---|
    Forward |     Reverse | ||
Biosynthetic genes: | |||
CHS | AB094986 | CAGAGATACCCAAAGGTTGGAAGGC | AACCATCCTTCCCGACAGCGAT |
ppa006888m | |||
CHI | DY634915 | TGAAGACCTCAAGGAACTTCTCAATGG | ACACAGGTGACAACGATACTGCCACT |
ppa011276m | |||
F3H | AB097151 | TCCGAGGGCAGAGCGAAGAAC | TTGTGGAGGCTTGTGAGGATTGG |
ppa007636m | |||
DFR | AB095030 | GGTCGTCCAGGTGAACATACTGCC | ATTTCTCATGCCATCCATGCCAC |
ppa008069m | |||
LDOX | EU292219 | AAGTGGGTCACTGCCAAGTGTGTTC | GTGGCTCACAGAAAACTGCCCAT |
ppa007738m | |||
UFGT | DN676790 | CCGCTGCCTCTCCCAACACTC | CCATCAGCCACATCAAACACCTTTAT |
ppa005162m | |||
ANR | AM288300 | ACTTCAAGGCTAAGGGGCTGCTG | CCAAGCCAGATAAACGCCAATCAC |
ppa008295m | |||
LAR | AJ872926 | CATCCACGGGGAAATTCACCTG | ACCCTTCCCAGAGTTACCATCACTGA |
ppa007994m | |||
FLS1 | GU938685 | GTTTTCTGACGGCAACGTTACGAA | CCCAACCCTAGCGATAGGAGCC |
ppa008322m | |||
Regulators: | Â | Â | Â |
MYB10 | EU155160 | TGATTCCAAGGGTCCACGCTAAAA | CTGGTCTTGGGTTAGATGAAGAACTGC |
ppa016711m | |||
bHLH3 | DY642427 | TTCCTCTACTAGACGGCGTCGTCG | GGAGGAGGATGGTGGTTGTGGTC |
ppa002884m | |||
WD40 | ACQ65867 | CCCAGCCTGATACCCCTTTGCT | GTCGGCGAACGGATATCCAAAAT |
ppa008187m | |||
MYBPA1 | CV047374 | GACCCAAGCACCCACAAGAAATTATC | GGCTTTAGTGGCTCCACATGTTGA |
ppa009439m | |||
MYB15 | ppa004560m | GGAAAATTGACACCTTCAGAAGGCC | TCTACCGCCTCTTCGCTTGGAA |
MYB16 | ppa010277m | AGCTTTTGACCAGAGGTATCGACCC | TGCCCAGAAGCCCATTAATAATGC |
MYB111 | ppa010716m | CGCTTATTGCTGGAAGGTTGCC | GCCTATGGTTATTGGGATCAATGCC |
MYB123 | ppa023768m | ACTCAACCGTCGACCAACATTGC | TCGGCGTGGTGAGGAAAGATG |
Reference gene: | |||
PpActin | BU045718 | GGAAATTACTGCATTAGCACCCAGC | CCAGATTCATCATACTCGGCTTTGG |
ppa007214m | |||