Figure 4

Quantitative real time-PCR results. A, the mRNA transcript level of the AtPAD4 gene in the overexpressing roots and empty vector (control) and the non-target Ubiquitin-3 gene transcripts. The x-axis represents the experiment type. The y-axis represents the absolute quantification of the mRNA transcript of different genes (number of target molecules), B, Showing the presence of the AtPAD4 insert in transgenic line, C, Showing the presence of the non-target Ubiquitin-3 gene transcripts, M, is molecular weight standard, R1-6, represents PCR amplicons from RNA extracted from individual roots.