Figure 6
Expression patterns of PR1 , PR2 , PR5 , PDF1.2 , ABA1 , COI1 and HSR203J genes after inoculation with Pst T1 + Pstab extracts in N. benthamiana . The expression level of PR1, PR2, PR5, PDF1.2, ABA1, COI1 and HSR203J was determined by qRT-PCR after inoculation of Pst T1 with MG medium extracts or Pstab extracts in N. benthamiana. Gene expression analyses were performed using three biological replications, where each biological replicate consists of three technical replicates. Bars represent the means ± standard deviation (SD).). Same letters above bars indicate no statistically significant difference (P<0.05) among plant genotypes for a given time point using one-way ANOVA followed by LSD test analysis. The qRT-PCR data were normalized to NbActin transcript and shown as relative to that of target gene expressions in 0 hr N. benthamiana leaves without any treatment.