Figure 2
Suppression of stomatal defense by the metabolite extract from Pstab supernatant in N. benthamiana . (A) Suppression of stomatal closure by Pstab extracts in N. benthamiana epidermal peels. KCl-MES; stomata opening buffer, MG ext/Pst T1; bacterial suspension of Pst T1 with MG media extracts, and Pstab ext/Pst T1; bacterial suspension of Pst T1 with Pstab extracts. (B) Ability of Pst T1 migration through stomata and a number of bacterial cells of Pst T1 in apoplastic space. Detached leaves of N. benthamiana were floated on Pst T1 suspension (1×107 CFU/ml) along with MG media, Pstab supernatant, and Pstab cell pellet extracts, and samples were collected at 1 hr and 4 hrs after incubation. Bars represent the means ± standard deviation (SD) (P<0.05, student’s t-test).