The effect of ‘Candidatus Liberibacter asiaticus’ infection on the proteomic profiles and nutritional status of pre-symptomatic and symptomatic grapefruit (Citrus paradisi) plants

Table 3 Citrus grapefruit leaf proteins that were up-accumulated in response to Las-infection

Spot^a	ASV^b	Protein function/name^c	Accession #	Theoretical^d		S^e	M^f	E^g
Spot^a	UP IP US IS	Protein function/name^c	Accession #	M _r	pI	S^e	M^f	E^g
		*Energy/Metabolisms*
29ⁱ		Granule-bound starch synthase	gi\|223029784	67320	8.56
33ⁱ		Granule-bound starch synthase	gi\|223029784	67320	8.56
61ⁱ		Granule-bound starch synthase	gi\|223029784	67320	8.56
		*Redox homeostasis*
34ⁱ		Peroxiredoxins, prx-1, prx-2, prx-3, putative	gi\|255578581	29299	8.38
119ⁱ		Cu/Zn superoxide dismutase	gi\|2274917	12784	5.82
147ⁱ		2Fe-2S ferredoxin-like protein	gi\|18397961	17602	7.75
		*Pathogen response*
14ⁱ		Chloroplastic light/drought-induced stress protein	gi\|22261807	35216	5.24
15ⁱ		Acidic class I chitinase	gi\|23496445	34123	4.70
19		Acidic class I chitinase	gi\|23496445	36735	4.81	64	8	24
39		Lectin-related protein precursor	gi\|11596188	29272	5.10	72	7	32
41^h		Chitinase	gi\|1220144	32459	5.06	53	5	20
43		Chitinase	gi\|1220144	36735	4.81	73	11	41
44		Lectin-related protein precursor	gi\|11596188	29272	5.10	85	7	32
66ⁱ		Chitinase	gi\|1220144	31909	5.06
88ⁱ		Chitinase	gi\|1220144	31909	5.06
92ⁱ		Chitinase	gi\|1220144	31909	5.06
124		Chitinase	gi\|1220144	36735	4.81	110	11	45
141ⁱ		CAP160 protein	gi\|22327778	65970	5.07
153		Putative miraculin-like protein 2	gi\|119367468	17806	6.74	105	7	49
156ⁱ		Miraculin-like protein 2	gi\|11596180	25652	6.10
160ⁱ		Chitinase	gi\|1220144	31909	5.06
179ⁱ		PR-4 type protein	gi\|3511147	15227	5.50
181ⁱ		Miraculin-like protein 2	gi\|87299377	24120	5.61
187ⁱ		Miraculin-like protein 2	gi\|87299377	24120	5.61
202		Putative miraculin-like protein 2	gi\|119367468	23610	8.18	126	9	54
203ⁱ		Miraculin-like protein 2	gi\|87299377	24120	5.61

^a The spot numbers correspond to the numbers given in Figure 2 and Additional file 3: Figure S2.
^b Average spot volume per treatment group; UP, uninfected reference for pre-symptomatic plants; IP, infected pre-symptomatic plants; US, uninfected reference for symptomatic plants; IS, infected symptomatic plants. Average spot volumes separated by letters to show significant difference are presented in Additional file 6: Appendix S1.
^c Protein function/name was determined by http://www.ncbi.nlm.nih.gov/BLAST/.
^d Theoretical relative molecular weight (M_r) and isoelectric point (pI) were calculated by http://www.expasy.org/. Observed M_r and pI can be extrapolated from Figure 1.
^e Mascot score of protein hit.
^f Number of matched peptide masses. The sequences of PMF-matched peptides per spot are provided in Additional file 7: Appendix S2.
^g Percent sequence coverage of matched peptides.
^h Protein identification confirmed by MALDI-TOF-MS/MS. Sequencing information is provided in Additional file 7: Appendix S2.
ⁱ Protein identification confirmed by LC-MS/MS.

ISSN: 1471-2229