The effect of ‘Candidatus Liberibacter asiaticus’ infection on the proteomic profiles and nutritional status of pre-symptomatic and symptomatic grapefruit (Citrus paradisi) plants

Nwugo, Chika C; Lin, Hong; Duan, Yongping; Civerolo, Edwin L

doi:10.1186/1471-2229-13-59

Table 2 Photosynthesis- and Energy/Metabolisms-related citrus grapefruit leaf proteins that were down-accumulated in response to Las-infection

From: The effect of ‘Candidatus Liberibacter asiaticus’ infection on the proteomic profiles and nutritional status of pre-symptomatic and symptomatic grapefruit (Citrus paradisi) plants

Spot^a	ASV^b	Protein function/name^c	Accession #^c	Theoretical^d		S^e	M^f	E^g
Spot^a	UP IP US IS	Protein function/name^c	Accession #^c	M _r	pI	S^e	M^f	E^g
		CO ₂ *assimilation/Photosynthesis*
3		Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit(chloroplast)	gi\|114329664	53950	6.19	143	19	41
28		Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit(chloroplast)	gi\|114329664	53950	6.19	94	14	36
49		Chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase activase large protein isoform	gi\|115334977	41738	5.07	150	24	56
67		Oxygen evolving enhancer protein 1	gi\|326467059	29262	5.32	133	14	67
71		Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplast precursor, putative	gi\|255584538	51073	5.33	145	20	50
72		Ribulose bisphosphate carboxylase/oxygenase activase 1, chloroplast precursor, putative	gi\|255584538	47200	5.94	144	21	55
91		Oxygen evolving enhancer protein 1	gi\|326467059	29262	5.32	134	15	70
101		Chloroplast ribulose-1,5-bisphosphate carboxylase/oxygenase activase small protein isoform	gi\|115334975	47200	5.94	148	26	63
113		Rubisco subunit binding-protein beta subunit, putative	gi\|255564820	65086	5.85	211	25	50
126		Carbonic anhydrase, putative	gi\|255568812	28499	5.51	135	13	56
159		Carbonic anhydrase 1	gi\|30678350	28483	6.39	78	8	38
164		Photosystem II stability/assembly factor HCF136, chloroplast precursor, putative	gi\|255559812	45094	8.46	147	10	38
180^h		PSI 9 kDa protein	gi\|224365649	9545	6.67	51	3	43
208		Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit (chloroplast)	gi\|114329664	53950	6.19	106	17	41
211		Ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit	gi\|24940138	20521	9.16	92	8	41
214^h		Oxygen-evolving enhancer protein 2, chloroplastic	gi\|225446775	26777	8.63	48	6	32
		*Energy/Metabolisms*
20		Protein grpE-like	gi\|225439145	24749	4.61	67	8	47
70		Sedoheptulose-1,7-bisphosphatase, chloroplast, putative	gi\|255579134	42768	5.82	67	15	31
75		Beta-tubulin	gi\|223018283	50941	4.76	197	27	60
78		Alpha-tubulin	gi\|134035496	42534	5.82	102	15	59
95		Caffeoyl CoA O-methyltransferase 1	gi\|229368458	20972	5.39	74	8	57
103		Phosphoribulose kinase, putative	gi\|255555933	45558	5.97	141	17	61
106		ATP synthase beta subunit, putative	gi\|255582911	59862	6.06	152	22	51
112		Transitional endoplasmic reticulum ATPase, putative	gi\|255556938	90244	5.14	265	27	47
122^h		Bis(5'-adenosyl)-triphosphatase-like	gi\|356539734	16962	6.07	48	4	40
130		Pyruvate dehydrogenase, putative	gi\|255543140	39870	5.95	67	11	29
132		Alcohol dehydrogenase, putative	gi\|255568816	41894	8.77	141	11	42
134		ATP synthase beta subunit, putative	gi\|255582911	59862	6.06	278	27	63
139		2-phospho-D-glycerate hydrolase	gi\|289600010	48059	5.54	142	15	53
154		Carboxymethylenebutenolidase, putative	gi\|255567721	30236	7.10	74	7	37
167		Phosphoglycerate kinase, putative	gi\|255544584	38338	9.37	108	10	38
171		Sorbitol dehydrogenase-like protein	gi\|21553353	39923	6.33	119	11	36
172		2-phospho-D-glycerate hydrolase	gi\|289600010	48059	5.54	235	20	64
173		Putative 2-3 biphosphoglycerate mutase	gi\|239056191	61119	5.65	170	16	42
186		Triosphosphate isomerase-like protein type II	gi\|262410515	27160	5.74	150	11	65
191		Malate dehydrogenase	gi\|211906490	35859	6.10	196	19	66
199		Aldehyde dehydrogenase, putative	gi\|255540719	52969	5.92	198	19	52

^a The spot numbers correspond to the numbers given in Figure 2 and Additional file 3: Figure S2.
^b Average spot volume per treatment group; UP, uninfected reference for pre-symptomatic plants; IP, infected pre-symptomatic plants; US, uninfected reference for symptomatic plants; IS, infected symptomatic plants. Average spot volumes separated by letters to show significant difference are presented in Additional file 6: Appendix S1.
^c Protein function/name and accession number was determined by http://www.ncbi.nlm.nih.gov/BLAST/.
^d Theoretical relative molecular weight (M_r) and isoelectric point (pI) were calculated by http://www.expasy.org/. Observed M_r and pI can be extrapolated from Figure 1.
^e Mascot score of protein hit.
^f Number of matched peptide masses. The sequences of PMF-matched peptides per spot are provided in Additional file 7: Appendix S2.
^g Percent sequence coverage of matched peptides.
^h Protein identification confirmed by MALDI-TOF-MS/MS. Sequencing information is provided in Additional file 7: Appendix S2.
ⁱ Protein identification confirmed by LC-MS/MS.

Back to article page

ISSN: 1471-2229

Contact us

General enquiries: journalsubmissions@springernature.com

BMC Plant Biology

Contact us