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Table 2 Degenerate primers for cloning of energy-related genes in litchi pericarp

From: Energy status of ripening and postharvest senescent fruit of litchi (Litchi chinensis Sonn.)

Gene Forward primer (5’ to 3’) Reverse primer (5’ to 3’) Product (bp)
AtpB GGGCCGTGGCCatgwsngcnac TCGGGCAGGCCGtcnarytcncc 1188
AOX1 GGAGGACGGCACCGAntggmmntg GCGTCCTTGGGCAGCCkccartartcda 676
UCP1 ACCATCGCCCGGGARGARGGNNT AGGTGCCCAGCCGGSYRAARTTNGG 652
AAC1 GCCGCCGCCccnathgarmg CGCCGCCGGAGCcrtayttyttnc 850
SnRK2 GGCGCCGGCAACttyggngtngc AGCTCCCTGGGCAGGttyttnarraac 773
  1. AtpB, ATP synthase β subunit; AOX1, alternative oxidase 1; UCP1, uncoupling mitochondrial protein 1; AAC1, ADP/ATP carrier 1; SnRK2, sucrose non-fermenting-1-related kinase 2.