Table 2 Degenerate primers for cloning of energy-related genes in litchi pericarp
From: Energy status of ripening and postharvest senescent fruit of litchi (Litchi chinensis Sonn.)
Gene | Forward primer (5’ to 3’) | Reverse primer (5’ to 3’) | Product (bp) |
|---|---|---|---|
AtpB | GGGCCGTGGCCatgwsngcnac | TCGGGCAGGCCGtcnarytcncc | 1188 |
AOX1 | GGAGGACGGCACCGAntggmmntg | GCGTCCTTGGGCAGCCkccartartcda | 676 |
UCP1 | ACCATCGCCCGGGARGARGGNNT | AGGTGCCCAGCCGGSYRAARTTNGG | 652 |
AAC1 | GCCGCCGCCccnathgarmg | CGCCGCCGGAGCcrtayttyttnc | 850 |
SnRK2 | GGCGCCGGCAACttyggngtngc | AGCTCCCTGGGCAGGttyttnarraac | 773 |