Table 2 Molecular markers and the phenotypic variation they explain in the two F 2 populations studied
From: Genetic analysis of tolerance to Boron toxicity in the legume Medicago truncatula
Marker | Marker, Mt chromosome | Primer sequence (forward and reverse, 5’ – 3’) | Restriction enzyme | Expected CAPS fragment size [bp] | Phenotypic variation explained [%] | ||
|---|---|---|---|---|---|---|---|
tap | Par/Cal | tx P* | tx C** | ||||
MTIC348 | SSR, chr. 4 | TGGAGGAGGGGTAGGATAGG ATGATGATGAGGCGGAGAAG | - | - | - | 95 | 94 |
h2_13f22h | SSR, chr. 4 | GGCTTCCTGATGCTGGTTAG ACAAGCAGGTTGGACACACA | - | - | - | 95 | 94 |
h2_6e22a | SSR, chr. 4 | CCATGGTGTCATTTGATCCT CCATGGTGTCATTTGATCCT | - | - | - | 96 | n.p. |
MtBtol-1 | CAPS (gene), chr. 4 | CTCTTTACCTCCCCCTCCTG TTGAACTACGATCAGGGTCGTTCCTA | ApoI | 387 | 170/217 | 96 | 94 |
MtBtol-2 | CAPS (gene), chr. 4 | TTGAACTACGATCAGGGTCGTTCCTA TGCTCCAGCACATCCAATTA | DraI | 102/131 | 102/256 | 96 | 94 |
256/280 | 411 | ||||||
MtBtol-3 | CAPS (gene), chr. 4 | TCCCACTGAAAATCGATTCAA GGCCAAAGCTCATTCCATT | MspI | 125/450 | 575 | 96 | 94 |
h2_21b19a | SSR, chr. 4 | GGAACACCGGACACAATACC CACTTCTCAGGCACAGCAAA | - | - | - | 96 | n.p. |
001D02 | SSR, chr. 4 | TCCCAACGCTTTTTCATTTC GAACTTGAAGAAGAACGCCG | - | - | - | 94 | 89 |
MtChr7 | CAPS, chr. 7 | TTGGAGCATTTATACCAGCAA TTGTATGCATCGGAGACTGC | NlaIII | 306 | 84/222 | n.a. | n.a. |
002G04 | SSR, chr. 1 | AGGGTCGCCTCAACTATTA TCAACACCATTTTCTCAATG | - | - | - | n.a. | n.a. |
002G02 | SSR, chr. 1 | TAACGTTACTCCCTCCTCCG TCTCCAACGAAGTTCAAGGG | - | - | - | n.a. | n.a. |
h2_13c11a | SSR, chr. 1 | TCACAACCTTCTTGCACCAG TCATAACTTGACCTGCACCA | - | - | - | n.a. | n.a. |