Figure 7From: CbCTB2, an O-methyltransferase is essential for biosynthesis of the phytotoxin cercosporin and infection of sugar beet by Cercospora beticolaColonization and penetration of sugar beet leaves by Ahlburg wild type DsRed and Ahlburg ctb2 disruption strain 2-1 -DsRed. Fluorescence microscopy was performed with a Zeiss fluorescence microscope, using the DsRed filter for detection of the hyphae and UV light to excite blue autofluorescence of the plant cell wall. At 4 days after inoculation, A Ahlburg wild type-DsRed mycelia and B Ahlburg ctb2 disruption strain 2-1-DsRed mycelia were visible on the surface of leaves. C At 4 days after inoculation, the wild type approached stomata and began to penetrate. D The gene disruption strain grew on the surface without entering through stomata at 4 days after infection. Scale bars: A and B = 100 μm, C and D = 20 μm. Abbreviations: ew epidermal cell wall, h hyphae, s stomata, sp stomatal pore, t trichome.Back to article page