Figure 1

Phylogenetic comparison of safflower CtFAD2 gene family and orthologous FAD2s from other plants. The phylogenetic tree was generated by Vector NTI (invitrogen). Included in the alignment were FAD2 desaturases (DES), hydroxylases (OH), epoxygenases (EPOX), acetylenases (ACET), and conjugases (CONJ). The GenBank accession numbers of the amino acid sequences represented in the phylogenetic tree are: atDES, AAM61113.1; lcDES, ACR15954.1; pfOH:DES, AAC32755.1; plOH, ABQ01458.1; coCONJ, AAK26632.1; haACET, ABC59684.1; rhACET, AAO38035.1; dsACET, AAO38036.1; caACET, ABC00769.1; cpEPOX, CAA76156.1; slEPOX, AAR23815.1; dcACET, AAO38033.1; dcDES:OH, AAK30206.1; fvACET,AAO38034.1; hhACET, AAO38031.1; boDES, AAC31698.1; haDES-2, AAL68982.1; haDES-3, AAL68983.1; haDES-1, AAL68981.1; ntDES, AAT72296.2; oeDES, AAW63040; siDES, AAF80560.1; ghDES-1, CAA65744.1; ptDES, XP_002297660.1; rcOH, AAC49010.1; cpDES, AAS19533.1; ghDES-4, AAQ16653.1; ghDES-2, CAA71199.1; jcDES, ADB93805.1; luDES, ACF49507.1. (at, Arabidopsis thaliana; lc, Lepidium campestre; pf, Physaria fendleri; pl, Physaria lindheimeri; co, Calendula officinalis; ha, Helianthus annuus; rh, Rudbeckia hirta; ds, Dimorphotheca sinuate; ca, Crepis alpine; cp, Crepis palaestina; sl, Stokesia laevis; dc, Daucus carota; fv, Foeniculum vulgare; hh, Hedera helix; Bo, Borago officinalis; nt, Nicotiana tabacum; oe, Olea europaea; si, Sesamum indicum; gh, Gossypium hirsutum; pt, Populus trichocarpa; rc, Ricinus communis; cp, Cucurbita pepo; jc, Jatropha curcas; lu, Linum usitatissimum).