Knock-out mutants for RAP2.6 . A: The T-DNA insertion (inverted triangle) in rap2.6-1 (GK_053G11 .01) and rap2.6-2 (GK_053G11 .02) is located in the 5´UTR. B: PCR with DNA from homozygous mutants and Columbia (Col). C: qRT-PCR for measurement of the expression of mutants as compared with WT in 14-d-old seedlings. For. primer and Rev. primer indicate the positions for the forward and reverse primer used for qRT-PCR.