Figure 10

Nematode resistance test. The resistance of overexpression lines and knock-out mutants of RAP2.6 was compared to wild type plants after infection with H. schachtii. A: Number of male and female nematodes per cm of root length calculated at 15 dpi setting the wild type as 100%. The statistical significance was determined by three independent replicates. Values are means ± SE, n = 15. The bar shows standard error for the mean and different letters indicate significante differences (P < 0.05; ANOVA and LSD). B: Size of female syncytia and female nematodes at 14 dpi. Ten syncytia were selected randomly from three independent replicates (total = 30) and the size of syncytia and associated female nematodes was determined. Data were analysed for significance difference using ANOVA (P < 0.05) and LSD. Values are means ± SE. C: Representive pictures of the photographed syncytia and nematodes from wild type Col and overexpression line OE 2–4. Scale bar (blue) = 100 μm.