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Figure 10 | BMC Plant Biology

Figure 10

From: Overexpression of the transcription factor RAP2.6 leads to enhanced callose deposition in syncytia and enhanced resistance against the beet cyst nematode Heterodera schachtiiin Arabidopsis roots

Figure 10

Nematode resistance test. The resistance of overexpression lines and knock-out mutants of RAP2.6 was compared to wild type plants after infection with H. schachtii. A: Number of male and female nematodes per cm of root length calculated at 15 dpi setting the wild type as 100%. The statistical significance was determined by three independent replicates. Values are means ± SE, n = 15. The bar shows standard error for the mean and different letters indicate significante differences (P < 0.05; ANOVA and LSD). B: Size of female syncytia and female nematodes at 14 dpi. Ten syncytia were selected randomly from three independent replicates (total = 30) and the size of syncytia and associated female nematodes was determined. Data were analysed for significance difference using ANOVA (P < 0.05) and LSD. Values are means ± SE. C: Representive pictures of the photographed syncytia and nematodes from wild type Col and overexpression line OE 2–4. Scale bar (blue) = 100 μm.

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