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Table 3 Secondary metabolites specifically accumulated in heat treated pericarp

From: Comparative proteomic and metabolomic profiling of citrus fruit with enhancement of disease resistance by postharvest heat treatment

Secondary metabolites

2 h

1 d

6 d

32 d

Quercetin-dihexose-deoxyhexose

16.71

14.81

15.42

n.s.

Hesperetin

12.32

2.08

n.s.

n.s.

Naringenin chalcone-hexose

15.04

n.s.

4.77

n.s.

Vanillic acid

14.73

n.s.

n.s.

14.37

Hydroxybenzoic acid-hexose

14.99

n.s.

n.s.

n.s.

Diosmin

4.06

n.s.

n.s.

n.s.

Rutin

3.69

n.s.

n.s.

n.s.

Phloretin-C-diglycoside

14.97

n.s.

0.06

n.s.

Chlorogenic acid

15.96

0.06

0.12

n.s.

Isosakuranetin

n.s.

16.42

n.s.

n.s.

Qucercetin

n.s.

16.03

0.14

0.12

Neohesperidin

n.s.

1.31

0.14

n.s.

Protocatechuic acid

n.s.

0.09

n.s.

n.s.

Caffeic acid hexose

n.s.

0.06

0.20

15.90

Naringenin

n.s.

n.s.

15.31

n.s.

Caffeic acid

n.s.

n.s.

0.09

n.s.

Hydroxylated naringenin-hexose

n.s.

n.s.

0.10

n.s.

Sinensetin

n.s.

n.s.

0.08

n.s.

p-Coumaroyl quinic acid

n.s.

n.s.

0.07

n.s.

Feruloylquinic acid

n.s.

n.s.

0.06

n.s.

Pantothenic acid -hexose

n.s.

n.s.

0.06

n.s.

Quercetin-O-dihexoside

0.08

n.s.

1.13

0.46

Cinnamic acid

0.11

n.s.

n.s.

n.s.

Eriodictyol-O-dihexoside

0.06

n.s.

n.s.

0.06

Ferulic acid

0.42

n.s.

0.09

n.s.

Sinapic acid

0.05

n.s.

0.07

n.s.

Jasmonic acid

0.07

n.s.

0.06

n.s.

  1. Samples at 2 h and 1, 6 and 32 DAT were used for the differential secondary metabolic profiling analysis using the HPLC-QTOF-MS instrument. Four replicates were compared and statistically analysed per sample using Student’s t-test; P < 0.05. After MS/MS analysis, a total of 58 metabolites were found to be significantly regulated (2-fold) in heat treated pericarp when compared to control pericarp (during the same period of storage), and 27 of these metabolites were identified. This table shows the ratio (HT/control during the same period of storage). n.s.: not significant.